我在BAM格式 一些芯片序列數據在某些時候,我想用本壘打做一個德從頭motif發現 findMotifsGenome.pl腳本HOMER從頭motif發現無法打開hg19 FASTA文件
問題似乎是這個應用程序無法打開refrence基因組fasta文件,即使它們是由應用程序本身安裝的!
有沒有人遇到過這個問題?
Linux命令使用:
$ perl的/home/chipseq_project/homer/bin/findMotifsGenome.pl /home/chipseq_project/homer/findpeak_output/peaks.txt hg19 /家庭/ chipseq_project /荷馬/ motif_output/- 給定大小
標準輸出文本:
Position file = /home/chipseq_project/homer/findpeak_output/peaks.txt
Genome = hg19
Output Directory = /home/chipseq_project/homer/motif_output/
Using actual sizes of regions (-size given)
Fragment size set to given
Found mset for "human", will check against vertebrates motifs
Peak/BED file conversion summary:
BED/Header formatted lines: 0
peakfile formatted lines: 7662
Peak File Statistics:
Total Peaks: 7662
Redundant Peak IDs: 0
Peaks lacking information: 0 (need at least 5 columns per peak)
Peaks with misformatted coordinates: 0 (should be integer)
Peaks with misformatted strand: 0 (should be either +/- or 0/1)
Peak file looks good!
Background fragment size set to 81 (avg size of targets)
Background files for 81 bp fragments found.
Extracting sequences from directory: /home/chipseq_project/homer/.//data/genomes/hg19//
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Not removing redundant sequences
Sequences processed:
0 total
Frequency Bins: 0.2 0.25 0.3 0.35 0.4 0.45 0.5 0.6 0.7 0.8
Freq Bin Count
Total sequences set to 50000
Choosing background that matches in CpG/GC Content...
非法除數爲零/home/chipseq_project/homer/bin/assignGeneWeights.pl線63 裝配序列文件... 正火低ORD使用homer2
Reading input files...
0 total sequences read
Autonormalization: 1-mers (4 total)
A inf% inf% -nan
C inf% inf% -nan
G inf% inf% -nan
T inf% inf% -nan
Autonormalization: 2-mers (16 total)
AA inf% inf% -nan
CA inf% inf% -nan
GA inf% inf% -nan
TA inf% inf% -nan
AC inf% inf% -nan
CC inf% inf% -nan
GC inf% inf% -nan
TC inf% inf% -nan
AG inf% inf% -nan
CG inf% inf% -nan
GG inf% inf% -nan
TG inf% inf% -nan
AT inf% inf% -nan
CT inf% inf% -nan
GT inf% inf% -nan
TT inf% inf% -nan
Autonormalization: 3-mers (64 total)
Normalization weights can be found in file: /home/chipseq_project/homer/motif_output//seq.autonorm.tsv
Converging on autonormalization solution:
...............................................................................
Final normalization: Autonormalization: 1-mers (4 total)
A inf% inf% -nan
C inf% inf% -nan
G inf% inf% -nan
T inf% inf% -nan
Autonormalization: 2-mers (16 total)
AA inf% inf% -nan
CA inf% inf% -nan
GA inf% inf% -nan
TA inf% inf% -nan
AC inf% inf% -nan
CC inf% inf% -nan
GC inf% inf% -nan
TC inf% inf% -nan
AG inf% inf% -nan
CG inf% inf% -nan
GG inf% inf% -nan
TG inf% inf% -nan
AT inf% inf% -nan
CT inf% inf% -nan
GT inf% inf% -nan
TT inf% inf% -nan
Autonormalization: 3-mers (64 total)
Finished preparing sequence/group files
----------------------------------------------------------
Known motif enrichment
Reading input files...
0 total sequences read
264 motifs loaded
Cache length = 11180
Using binomial scoring
Checking enrichment of 264 motif(s)
|0% 50% 100%|
非法司/home/chipseq_project/homer/bin/findKnownMotifs.pl線142 呃寡零------------------ ---------------------------------------- De novo motif finding(HOMER)
Scanning input files...
!!!有什麼不對......你確定你選擇了正確的長度來尋找主題嗎? !!!即也檢查你的序列文件!
Scanning input files...
!有什麼不對......你確定你選擇了正確的長度來尋找主題嗎? !!!即也檢查你的序列文件!
-blen automatically set to 2
Scanning input files...
!有什麼不對......你確定你選擇了正確的長度來尋找主題嗎? !!!即也檢查你的序列文件! 在/home/chipseq_project/homer/bin/compareMotifs.pl 1289行的數字gt(>)中使用未初始化的值。 !!!過濾掉所有圖案! 工作完成 - 如果結果看起來不錯,請發送啤酒..
Cleaning up tmp files...
所以,你問堆棧溢出調試別人的腳本,但沒有實際提供腳本?對不起,這有點超出了SO的範圍。如果您可以提供重現問題所需的代碼片段,我們可能會有機會 – Sobrique