邊緣多彩MDS陰謀R

我有問題在邊緣R中創建MDS陰謀，以可視化實驗（白血病）和控制（健康捐助者）羣體的顏色。邊緣多彩MDS陰謀R

我用htseq文件作爲edgeR的輸入。每個文件由兩列組成 - gene_ID和讀取計數。「A」代表白血病患者，「H」代表健康捐獻者。

這裏是我的代碼：

創建一個表：

samples <- matrix(c("A18.txt","experiment","blood_exp", 
     "A19.txt","experiment","blood_exp", 
     "A20.txt","experiment","blood_exp", 
     "A23.txt","experiment","blood_exp", 
     "A24.txt","experiment","blood_exp", 
     "A26.txt","experiment","blood_exp", 
     "A30.txt","experiment","blood_exp", 
     "A37.txt","experiment","blood_exp", 
     "H11.txt","control","blood_control", 
     "H12.txt","control","blood_control", 
     "H13.txt","control","blood_control", 
     "H15.txt","control","blood_control", 
     "H16.txt","control","blood_control", 
     "H17.txt","control","blood_control", 
     "H18.txt","control","blood_control", 
     "H19.txt","control","blood_control"), 
    nrow = 16, ncol = 3, byrow = TRUE, dimnames = list(c(1:16), c("library_name","condition","group_ALL_vs_control"))) 

samples <- as.data.frame (samples, row.names = NULL, optional = FALSE, stringAsFactors = default.stringAsFactors())

使用磨邊機功能，readDGE，在READS COUNT文件創建frou htseq數爲：

counts <- readDGE(samples$library_name, path = 'C:/Users/okbm4/Desktop/htseq_files', columns=c(1,2), group = samples$group_ALL_vs_control, header = FALSE) 

colnames(counts) <- samples$library_name

過濾器弱表達和無信息（即amibigous）功能：

noint <- rownames(counts) %in% c('__no_feature','__ambiguous','__too_low_aQual','__not_aligned','__alignment_not_unique') 

cpms <- cpm(counts) 
keep <- rowSums (cpms > 1) >= 4 & !noint 
counts <- counts[keep,]

創建DGElist對象

counts <- DGEList(counts=counts,group = samples$group_ALL_vs_control)

估計歸一化因子，這是對文庫大小

counts <- calcNormFactors(counts)

檢查使用MDS情節樣本之間的關係正常化。

pdf(file = 'HCB_ALL.pdf', width = 9, height = 6) 

plotMDS(counts, labels = c('A18.txt','A19.txt','A20.txt','A23.txt','A24.txt','A26.txt','A30.txt','A37.txt','H11.txt','H12.txt','H13.txt','H15.txt','H16.txt','H17.txt','H18.txt','H19.txt'), 

xlab = 'Dimension 1', 
ylab = 'Dimension 2', 
asp = 6/9, 
cex = 0.8, 

main = 'Multidimentional scaling plot') 
par(cex.axis =0.6, cex.lab = 0.6, cex.main = 1)

我附加了以前生成的文件。