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我已經創建了一個腳本，該腳本可成功搜索XML格式的Blastx輸出文件中的關鍵字（由用戶指定）。現在，我需要將包含對齊標題中關鍵字的記錄（查詢，命中，分數，evalue等）寫入新文件。從blastx輸出文件中提取特定條目，寫入新文件
我已經爲每個查詢標題，命中標題，e值和對齊長度創建了單獨的列表，但似乎無法將它們寫入新文件。
問題1：如果Python錯誤和其中一個列表缺少一個值會怎麼樣...？然後，所有其他列表將提供有關查詢的錯誤信息（「行滑移」，如果您願意......）。
問題2：即使Python沒有錯誤，並且所有列表長度相同，我如何將它們寫入文件以便每個列表中的第一項相互關聯（並因此，每個列表中的項目＃10也是相關的？）我應該創建一個字典嗎？
問題3：字典只有一個鍵值，如果我的查詢有幾個不同的命中？不知道它是否會被覆蓋或跳過，或者只是錯誤。有什麼建議麼？我現在的腳本：
from Bio.Blast import NCBIWWW from Bio.Blast import NCBIXML import re #obtain full path to blast output file (*.xml) outfile = input("Full path to Blast output file (XML format only): ") #obtain string to search for search_string = input("String to search for: ") #open the output file result_handle = open(outfile) #parse the blast record blast_records = NCBIXML.parse(result_handle) #initialize lists query_list=[] hit_list=[] expect_list=[] length_list=[] #create 'for loop' that loops through each HIGH SCORING PAIR in each ALIGNMENT from each RECORD for record in blast_records: for alignment in record.alignments: #for description in record.descriptions??? for hsp in alignment.hsps: #for title in description.title??? #search for designated string search = re.search(search_string, alignment.title) #if search comes up with nothing, end if search is None: print ("Search string not found.") break #if search comes up with something, add it to a list of entries that match search string else: #option to include an 'exception' (if it finds keyword then DOES NOT add that entry to list) if search is "trichomonas" or "entamoeba" or "arabidopsis": print ("found exception.") break else: query_list.append(record.query) hit_list.append(alignment.title) expect_list.append(expect_val) length_list.append(length) #explicitly convert 'variables' ['int' object or 'float'] to strings length = str(alignment.length) expect_val = str(hsp.expect) #print ("\nquery name: " + record.query) #print ("alignment title: " + alignment.title) #print ("alignment length: " + length) #print ("expect value: " + expect_val) #print ("\n***Alignment***\n") #print (hsp.query) #print (hsp.match) #print (hsp.sbjct + "\n\n") if query_len is not hit_len is not expect_len is not length_len: print ("list lengths don't match!") break else: qrylen = len(query_list) query_len = str(qrylen) hitlen = len(hit_list) hit_len = str(hitlen) expectlen = len(expect_list) expect_len = str(expectlen) lengthlen = len(length_list) length_len = str(lengthlen) outpath = str(outfile) #create new file outfile = open("__Blast_Parse_Search.txt", "w") outfile.write("File contains entries from [" + outpath + "] that contain [" + search_string + "]") outfile.close #write list to file i = 0 list_len = int(query_len) for i in range(0, list_len): #append new file outfile = open("__Blast_Parse_Search.txt", "a") outfile.writelines(query_list + hit_list + expect_list + length_list) i = i + 1 #write to disk, close file outfile.flush() outfile.close print ("query list length " + query_len) print ("hit list length " + hit_len) print ("expect list length " + expect_len) print ("length list length " + length_len + "\n\n") print ("first record: " + query_list[0] + " " + hit_list[0] + " " + expect_list[0] + " " + length_list[0]) print ("last record: " + query_list[-1] + " " + hit_list[-1] + " " + expect_list[-1] + " " + length_list[-1]) print ("\nFinished.\n")